Operation - VWR VisiScope 100POL Serie Manuel D'utilisation

Operation

Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the
lock-screw.
Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is centred
over the stage opening.
Lamp settings
The microscope is fitted with power white LED illuminator. Before turning on the light, read the section about
electrical safety precautions. Insert the plug of the 5Vdc power supply into the jack on the rear. Turn the
brightness adjustment knob in order to switch the light on and to reach a brightness suitable for observation.
Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary distance by
turning the two parts until one circle of light can be seen.
Focus and diopter adjustment
Rotate the coarse focusing knob to bring the slide into focus using an objective with a low magnifi¬cation.
Adjust the fine focusing knob to get the image sharp and clear while observing with your right eye, then turn
the dioptric adjustment ring on the left eyepiece until a sharp and clear image can be observed also with the
other eye. Turn the tension-adjust-knob to get a suitable tension for the focus system. When the image is in
focus, turn the nosepiece to choose the objective you need.
Make a polarization analysis
Insert both the swing-out polarizer filter and the analyzer filter (which is placed under the observation head).
Rotate now the polarizer filter until you have a dark background ("extinction condition"). By rotating the stage
it's possible now to observe the polarization effects of your sample. You can lock the position of the rotating
stage through the screw on the back of the stage
Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus controlling ima-
ge contrast and resolution.
INSERT THE POLARIZER
FILTER
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