Using The Microscope - Optika B-290 Serie Manuel D'instructions

4.0 USING THE MICROSCOPE

4.1 Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then
lock the lock-screw.
4.2 Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is
centred over the stage opening by adjusting the coaxial knobs of the stage.
4.3 Lamp settings
The microscope is fitted with power white LED illuminator. Before turning on the light, read the section
5.3 about electrical safety precautions. Insert the plug of the 6Vdc power supply into the jack on the
rear and turn on the ON/OFF switch. Turn the brightness adjustment knob to a brightness suitable for
observation.
4.4 Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary dis-
tance by turning the two parts until one circle of light can be seen.
4.5 Focus and diopter adjustment
Rotate the coarse focusing knob to bring the slide into focus using an objective with a low magnifica-
tion. Adjust the fine focusing knob to get the image sharp and clear while observing with your right
eye, then turn the dioptric adjustment ring on the left eyepiece until a sharp and clear image can be
observed also with the other eye. Turn the tension-adjust-knob to get a suitable tension for the focus
system. When the image is in focus, turn the nosepiece to choose the objective you need.
4.6 Condenser adjustment
Raise or lower the condenser through the knob to obtain a clear and uniform illumination of the sam
ple. To center the condenser: completely close the iris diaphragm. Using the condenser centering
screws, move the diaphragm in the center of the field of view. Then gradually expand the diaphragm
until it is tangent to the edges of the field of view. If necessary, you can perform an additional adjust
ment.
The condenser is centered when the edges of iris diaphragm are tangent to the field of view.
4.7 Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus control-
ling image contrast and resolution.
4.8 Photo and video capture
To use a camera, for photo or video capturing, connect it to the trinocular tube using the proper
adaptor. Please refer to the camera manuals for further details.
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