NAL NADAL Influenza A Test Instructions D'utilisation page 7

ENGLISH
 Do not use specimens contaminated by blood.
 Do not substitute or mix components from different test
kits.
 Do not eat, drink or smoke in the area where specimens
and test kits are handled.
 Wear protective clothing such as laboratory coats,
disposable gloves and eye protection when specimens are
being assayed.
 Handle all specimens as if they contain infectious agents.
Observe established precautions for microbiological risks
throughout all procedures and standard guidelines for the
appropriate disposal of specimens.
 The test kit contains products of animal origin. Certified
knowledge of the origin and/or sanitary state of the animals
does not completely guarantee the absence of transmissible
pathogenic agents. It is therefore recommended that these
products be treated as potentially infectious and handled in
accordance with usual safety precautions (e.g., do not
ingest or inhale).
 Humidity and temperature can adversely affect test results.
 Used testing materials should be disposed of according to
local regulations.
8. Specimen Collection and Preparation
Nasal swab specimen:
 Gently insert a sterile swab into the nostril.
 Collect secretion by rotating the swab several times against
the turbinates.
Pharyngeal swab specimen:
 Gently insert a sterile swab into the pharynx and collect
secretion by brushing the swab several times against the
reddened posterior pharyngeal wall and palatine tonsils. Be
careful not to collect saliva with the swab.
Nasal/nasopharyngeal aspirate specimen:
 To collect a nasal/nasopharyngeal aspirate specimen,
connect a catheter to the mucus trap attached to the
aspiration device. Insert the catheter through the nostril
and into the nasal cavity before starting the aspiration
device. Once the sample has been collected, dip a sterile
swab into the nasal/nasopharyngeal aspirate. Allow the
swab to absorb the specimen.
9. Test Procedure
Bring tests, specimens, buffer and/or controls to room
temperature (15-30°C) prior to testing.
1. Place a clean extraction tube into the
designated area of the reagent holder.
Holding the buffer bottle vertically and
without touching the edge of the tube,
add 8 drops (approximately 300 µL) to
the extraction tube.
2. Insert the swab with the collected
specimen into the tube. Swirl the swab
for approximately 10 seconds, pressing
it against the wall of the extraction
tube to extract the antigens contained
in the swab.
3. Remove the swab, squeezing it firmly
against the tube to release as much
nal von minden GmbH • Carl-Zeiss-Strasse 12 • 47445 Moers • Germany • info@nal-vonminden.com • www.nal-vonminden.com
NADAL® Influenza A/B Test
(Ref. 241015N-10)
liquid as possible. Dispose of the swab
in accordance with guidelines for the
handling of infectious agents.
4. Remove the test strip from the foil
pouch and use it as soon as possible.
The best results will be obtained if the
test is performed immediately after
opening the foil pouch. Label the test
strip with the patient or control
identification.
5. Holding the test strip at the marked
end with arrows pointing downwards,
immerse it vertically in the tube. In
order to avoid contamination, do not
touch the test strip membrane and
make sure not to immerse the test strip
beyond the maximum immersion mark
(MAX). Leave the test strip in the tube.
If the procedure is followed correctly,
the extracted specimen should be
below the maximum immersion mark
(MAX) on the test strip.
6. Start the timer.
7. Wait for the coloured line(s) to appear.
Read the test result after 8 minutes. Do
not interpret the result after more than
15 minutes.
10. Result Interpretation
Positive for Influenza A
A coloured line develops in the control
line region 'C' and another coloured line
develops in the test line region 'A'. The
result indicates that influenza virus type A
antigens have been detected in the
specimen.
Positive for Influenza B
A coloured line develops in the control
line region 'C' and another coloured line
develops in the test line region 'B'. The
result indicates that influenza virus type B
antigens have been detected in the
specimen.
Positive for Influenza A and Influenza B
In addition to the control line 'C', a
coloured line develops in the test line
region 'A' and another in the test line
region 'B'. The result indicates that
influenza virus type A and B antigens have
been detected in the specimen.
Note: The colour intensity in the test line region (A) and (B)
may vary depending on the influenza virus type A or B antigen
concentration in the specimen. Therefore, any shade of colour
in the test line region (A) or (B) should be considered positive.
Note that this is a qualitative test only and it cannot
determine the analyte concentration in the specimen.
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