Pipetting Techniques - Thermo Electron FINNPIPETTE COLOUR MULTICHANNELS Guide D'utilisation

ENGLISH
TIP EJECTION (Fig. 3)
Each pipette is fitted with a tip ejector system to help
eliminate the possibility of contamination. For tip ejection,
two methods are available:
A. If one-handed operation is preferred, twist the pipette
handle so that the tip ejector pusher and tip ejector on
the body assembly are in line. Press the tip ejector
pusher with your thumb directing the tips towards a
suitable waste receptable.
B. In two-handed operation, press the tip ejector on the
body assembly directing the tips towards a suitable
waste receptacle. With some tip types this may be an
easier and lighter method for user if the tips are very
tight.
Figures 4-6:
A =
B =
C =
FORWARD TECHNIQUE (Fig. 4)
Ensure that clean tips are firmly pushed on to the tip cones
of the pipette.
The operation of the pipette is controlled by the operating
button. For the best possible accuracy:
Operate the button slowly and with steady speed at all
times, particularly when working with liquids having high
viscosity. Never let the operating button snap back. When
working with serum or biological fluids prime the tips with
the liquid before pipetting. This is done by filling and
emptying the tips.
Hold the pipette vertically (10 degrees maximum from
vertical) during the liquid intake. For maximum hand
control, hold the pipette tightly in the hand with the
"Grippy" resting on the index finger.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the operating button to the first stop.
2. Dip the tips just under the surface of the liquid in the
reservoir (2-3 mm) and slowly release the operating
button. Withdraw the tips from the liquid touching them
against the edge the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the operating
button to the first stop. After a delay of about one
second, continue to depress the operating button all
the way down to the second stop. This action will
empty the tips.
4. Release the operating button to the ready position.
If necessary, change the tips and continue with the
pipetting.
REVERSE TECHNIQUE (Fig. 5)
The reverse technique is suitable for dispensing liquids
having high viscosity or a tendency to foam easily. The
technique is also recommended for dispensing very small
volumes. Fill a clean reagent reservoir with the liquid to be
dispensed.

PIPETTING TECHNIQUES

Ready position
First stop
Second stop
4